AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL KUMIS KUCING ( Orthosipon Aristatus )TERHADAP PERTUMBUHAN BAKTERI Staphyloccocus aureus DENGAN MENGGUNAKAN METODE DIFUSI
DOI:
https://doi.org/10.55606/klinik.v1i2.1176Keywords:
Cat's Whiskers Leaf, Staphyloccocus aureusAbstract
Cat's whiskers leaf plant (Ortosiphon aristatues.) is one of the plants that is efficacious as a medicinal plant. The part of the cat's whiskers leaf plant is used, namely the leaves that contain saponin compounds, flavonoids, alkaloids and tannins. The purpose of this study was to determine the most effective inhibition produced by the ethanol extract of the leaves of the cat's whiskers (Ortosiphon aristatues.) against the growth of Staphyloccocus aureus bacteria. The benefit of this research is to increase knowledge about the antimicrobial potential of ethanol extract of cat whiskers (Ortosiphon arietatus) leaves against the growth of Staphyloccocus aureus bacteria. This research method uses the diffusion method for wells. The sample used was cat whiskers leaf extract with pure strain of Staphyloccocus aureus. Treatment with cat whiskers leaf extract, the results of the test of the inhibitory activity of the cat whiskers leaf extract showed an average difference at a concentration of 65% with a diameter of 22 mm, a concentration of 70% with a diameter of 25 mm, and a concentration of 75% with a diameter of 30 mm. Ethanol extract of cat whiskers leaves with a concentration of 65%, 70%, and 75% effectiveness in inhibiting the growth of Staphylococcus aureus bacteria. Based on observations, it was shown that the ethanolic extract of Kumis Kucing leaves gave the most effective inhibitory concentration at a concentration of 75% against Staphyloccocus aureus bacteria. with a diameter of 30 mm
References
Agustiningtyas iren 2021. Sterilisasi, Laboratorium mikrobiologi universitas islam indonesia
Prasetyo dan Inoriah 2013. Pengelolaan Budidaya Tanaman Obat-obatan, cetakan ke -1, Bengkulu: badan penerbit fakultas pertanian UNIB, 2013
Anonim, tth, Bab II. Tinjauan Pustaka, http://repository.usu.ac.id /bitstream/ 123456789/52646/4/Chapter II.pdf, diakses pada tanggal 18 Februari 2021
Atingul Ma’rifah (2012) Efek Ekstrak Daun Sirih Merah (Piper crocatum) terhadap pertumbuhan bakteri Staphylococcus aureus. Universitas Islam Negeri hidayatullah.
Derryl A, Yuana. (2016). Gambaran Penggunaan Antibiotik Dengan Resep dan Tanpa Resep Dokter di beberapa Apotek di area Jember Kota. Fakultas Farmasi Universitas Jember.
Herbarium Bogoriense. Bogor. Indonesia. 86 hal. Kandou. F.E., S. Magenda dan S.D. Umboh ... Utami, N. 2014. Suku Balsaminaceae di Jawa: Status Taksonomi dan.5 halaman
Hossain, Mohammed Kawser, et al., 2016. Molecular Mechanisms of the AntiObesity and Anti-Diabetic Properties, of Flavonoids. Int J. Mol. Sci. (17). 569 : pp. 4-7
Istiqomah (2013) perbandingan metode ekstraksi maserasi dan sokletasi terhadap kadar piperin buah cabe jawa universitas syarif hidayatullah
Utomo, IndriRengganisBudi (2017) Efektivitas Ekstrak Etanol Daun Kumis Kucing (Orthosiphon Aristatus) Sebagai Antibakteri Terhadap Porphyromonas Gingivalissecara In Vitro. Sarjana thesis, Universitas Brawijaya.
Ncube, N. S., Afolayan A. J., Okoh A. I. (2011). Assessment Techniques of Antimikrobial Properties of Natural Compounds of plant Origin : Current Methods pof future Trends. African Journal of Biotechnology; 7 (12).
Nurhayati, L. S., Yahdiyani, N., & Hidayatulloh, A. (2020). Perbandingan pengujian aktivitas antibakteri starter yogurt dengan metode difusi sumuran dan metode difusi cakram. Jurnal Teknologi Hasil Peternakan, 1(2), 41-46.
Nurjanah, U. (2018). Efektivitas Perasan Daun Bahagia (Dieffenbachia bowmanii) Terhadap Pertumbuhan Bakteri Staphylococcus aureus (Doctoral dissertation, Universitas Muhammadiyah Surabaya).
Permana 2015. Kualitas ekstrak etanol 70 % daun kelor (moringga olifer A.L.) Dalam ramuan penambah asi.kefarmasian Indonesia.
Radji 2015. Analisis kandungan vitamin C dalam daun kelor (moringga olifer A.L.) dengan metode difusi agar fitofarmaka Indonesia.
Rukmana Maarif Rizal, (2015). Aktivitas Antibakteri dari Ekstrak Etanolik Daun Kumis Kucing (Orthosiphon stamineus) pada Bakteri Staphylococcus aureus. Universitas Setia Budi.
Saraswati 2015. Kandungan nutrisi dann sifat fungsional tanaman kelor (moringga olifer A.L.) Jakarta balai pengkajian teknologi pertanian Jakarta.